Test Directory

Home Test Search Results sIL-2RA Level (CD25)
sIL-2RA Level (CD25)

Justification

This test is used to detect soluble IL-2Rα in human plasma or serum produced in response to increased activation of B and T cells and immune system activation. Studies show elevated levels of sIL-2Rα in serum with the onset of rejection episodes in allograft recipients, autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus (SLE), hemophagocytic lymphohistiocytosis (HLH) and in the course of some leukemias and lymphomas. This test may be of diagnostic and/or prognostic value in HLH, granulomatous, autoimmune, and malignant diseases.

STAT: < 24 hours (7 days a week)

ELISA

Draw Tube: Purple Top

Sample Type: EDTA Plasma

Specimen Requirements

Sample Type Volume Required Minimum Volume Stability
PREFERRED EDTA Plasma 1mL 0.5mL Room Temp.: 3 days
Refrigerated: 3 days
Frozen (-20C): 1 month
Frozen (-80C): 6 months
ALTERNATIVE Serum 1mL - Room Temp.: 3 days
Refrigerated: 3 days
Frozen (-20C): 1 month
Frozen (-80C): 6 months
REJECTION CRITERIA Thawed in transit if shipped frozen, refrozen, clotted sample
SPECIAL INSTRUCTIONS -

General Information

METHODOLOGY ELISA
STAT TAT < 24 hours (7 days a week)
STAT TAT Performance > 90% of results released in 20 hours 7 days a week
ROUTINE TAT < 1 week
ALTERNATIVE NAMES Soluble IL-2 Receptor Alpha level, sIL2R
DESCRIPTION Chromogenic ELISA for the quantitative measurement of soluble IL-2Rα in human plasma and serum.
LIMITATIONS -
NORMAL RANGE EDTA Plasma: 214-1910 pg/mLSerum: 325-1785 pg/mL
ASSOCIATED TESTING -
REFERENCES

1. Waldmann TA (1993) Immunol. Today 14: 264.
2. Colvin RB et al. (1987) Clin. Immunol. Immunopathol. 43: 273.
3. Southern JF et al. (1987) J. Heart Transplant. 5:370.
4. Simpson MA 91991) Arch.Surg. 126: 717.
5. Wolf RE et al. (1988) Arthritis Rheum. 31: 729.
6. Pizzolo G et al. (1987) Br. J. Haematol. 67: 377.
7. Wagner DK et al. (1987) J. Clin. Oncol. 5:1262.
8. Semezato G et al. (1987) Blood 70:396.
9. Chilosi M et al. (1987) Blood 70:1530.
10. Harrington DS et al. (1988) Arch. Pathol. Lab. Med. 112: 597.
11. Steis RG et al. (1988) Blood 71:1304.
12. Damoiseaux (2020) Clinical Immunology 218, 108515.
SAMPLE REPORT Upon request
NEW YORK STATE APPROVED Yes

Test Codes

ORDER CODE P3391
CPT CODE 83520
LOINC CODE 44422-4